The purposes of this study were to evaluate the adherence of bacteria on various denture base resin materials and effects of chitosan, added to denture base materials on bacterial adherence. PMMA denture base resin such as heat-cured Vertex-RS, self-cured Vertex-SC and 4-META denture base resin such as heat-cured Meta-Dent self-cured Meta-Fast were used in this study. Samples were divided into two groups : the denture base resin with chitosan, without chitosan. Streptomccus mutans and Lactobacillus casei were used in this study. The surface of samples was observed by SEM.
When chitosan was added to M17 and MRS broth, viable cell count of bacteria was reduced. Viable cell count of Streptococcus mutans on the samples decreased as follows: Meta-Dent, Vertex-SC, Meta-Fast, Vertex-RS. Viable cell count of Lactobacillus, casei on the samples decreased as follows: Vertex-RS, Meta-Dent, Meta-Fast, Vertex-SC.
The resin with chitosan showed lower adherence of bacteria than without chitosan. The images of SEM shoed that the surface of the resin with chitosan was rougher than without chitosan.
These results showed that the denture base resin materials with chitosan have rougher surface than without chitosan, but less bacteria adhered on there.
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